蛋白名稱:PDCD1人重組蛋白，10377-H05H

蛋白構(gòu)建:A DNA sequence encoding the human PDCD1 (NP_005009.2) (Met1-Gln167) was expressed with the Fc region of mouse IgG1 at the C-terminus.

表達宿主:HEK293 Cells

蛋白純度:≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

蛋白活性:

1.Immobilized PD-1 Protein, Human, Recombinant (ECD, mFc Tag) (Cat:10377-H05H) at 2 μg/mL (100 μL/well) can bind PD-L1 Protein, Human, Recombinant (ECD, His Tag), Biotinylated (Cat:10084-H08H-B), the EC50 is 150-800 ng/mL.

2. Immobilized Recombinant Human PD1 / PDCD1 / CD279 Protein (ECD, Fc Tag) (Cat: 10377-H05H) at 2 μg/mL (100 μL/well) can bind Anti-PD1 Antibody, Human IgG4 (Nivolumab), the EC50 is 20-60 ng/mL (Routinely tested).

3.Immobilized Recombinant Human PD1 / PDCD1 / CD279 Protein (ECD, Fc Tag) (Cat: 10377-H05H) at 2 μg/mL (100 μL/well) can bind Recombinant Human B7-DC / PD-L2 / CD273 / PDCD1LG2 Protein (Fc Tag) (Cat: 10292-H02H) , the EC50 is 15-45 ng/mL (Routinely tested).

4.Loaded Recombinant Human PD-1 Protein, mFc Tag (Cat. No. 10377-H05H) on AMC Biosensor, can bind Recombinant Human PD-L2/B7-DC Protein, His Tag (Cat. No. 10292-H08H) with an affinity constant of 58.6 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).

蛋白內(nèi)毒素:< 1.0 EU per μg protein as determined by the LAL method.

預(yù)測N端:Leu 25

蛋白分子量:The recombinant human PDCD1 consists of 377 amino acids and predicts a molecular mass of 42.3 kDa. As a result of glycosylation, it migrates as an approximately 58.9 kDa band in SDS-PAGE under reducing conditions.

蛋白NP號:NP_005009.2

蛋白氨基酸序列:Met1-Gln167

蛋白標(biāo)簽:C-mouse IgG1-Fc

歡迎去義翹神州網(wǎng)查看PDCD1人重組蛋白，10377-H05H詳細(xì)信息。網(wǎng)址：https://cn.sinobiological.com/recombinant-proteins/human-pd-1-10377-h05h

義翹神州研發(fā)團隊擁有多年的重組蛋白研發(fā)經(jīng)驗。多年來，我們的團隊已開發(fā)出超過9300余種重組蛋白，涵蓋免疫檢查點、抗體藥物靶點、CAR-T細(xì)胞治療靶點、Fc受體、流感病毒蛋白以及細(xì)胞因子等熱門研究領(lǐng)域。義翹神州重組蛋白具有高純度、高生物活性和低內(nèi)毒素水平等特性，可為生物藥靶點發(fā)現(xiàn)、蛋白質(zhì)結(jié)構(gòu)和功能分析、細(xì)胞療法和重組酶酶學(xué)性質(zhì)等科學(xué)研究提供支持。

義翹神州利用蛋白表達平臺，提供重組蛋白生產(chǎn)"一站式"技術(shù)服務(wù)。我們的服務(wù)包括蛋白基因合成、蛋白質(zhì)密碼子優(yōu)化、蛋白質(zhì)表達載體設(shè)計、大規(guī)模細(xì)胞培養(yǎng)和發(fā)酵、蛋白純化和蛋白質(zhì)量控制檢測。我們在蛋白表達和大規(guī)模生產(chǎn)方面有著顯著的成本節(jié)約和速度優(yōu)勢，同時在設(shè)計各種類型的重組蛋白純化項目（帶或不帶純化標(biāo)簽）方面也擁有著豐富的經(jīng)驗和專業(yè)技術(shù)。 義翹神州擁有經(jīng)驗豐富的技術(shù)團隊，每年可研發(fā)生產(chǎn)1,000+種重組蛋白開發(fā)和大規(guī)模生產(chǎn)項目。我們有能力進行快速的蛋白質(zhì)表達和純化工藝開發(fā)，根據(jù)需求進行工藝放大研究，并且可滿足高通量和大規(guī)模批量生產(chǎn)蛋白質(zhì)的要求。 我們致力于成為重組蛋白和單克隆抗體大規(guī)模生產(chǎn)的實驗室，為藥物研發(fā)企業(yè)和生命科學(xué)機構(gòu)提供高水平的技術(shù)服務(wù)。

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