蛋白名稱:ACE2人重組蛋白，10108-H02H

蛋白構(gòu)建:A DNA sequence encoding the extracellular domain (Met 1-Ser 740) of human ACE2 precursor (NP_068576.1) was expressed with the fused Fc region of human IgG1 at the C-terminus.

表達(dá)宿主:HEK293 Cells

蛋白純度:≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

蛋白活性:

1.Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2 (2019-nCoV) Spike S1-His (Cat: 40591-V08B1) at 2 μg/mL (100 μL/well) can bind human ACE2 (hFc tag) (Cat: 10108-H02H), the * of ACE2 is 5-35 ng/mL.

2.Captured ACE2（10108-H02H on proA Chip can bind SARS-CoV-2 B.1.1.529 (Omicron) Spike S1 Protein (Cat.No.40591-V08H41) with an affinity constant of 11.78 nM as determined in a SPR assay (Biacore T200)(Routinely tested).

3.Captured ACE2（10108-H02H on anti-Human IgG Fc via CM5 Chip can bind SARS-CoV-2 B.1.1.529 (Omicron) Spike RBD Protein (Cat. No. 40592-V08H121) with an affinity constant of 5.667 nM as determined in a SPR assay (Biacore T200)(Routinely tested).

4.Loaded SARS-CoV-2 (2019-nCoV) Spike RBD-mFc Recombinant Protein (Cat. No. 40592-V05H) on AMC Biosensor, can bind Recombinant Human ACE2 Protein , hFc Tag (Cat. No. 10108-H02H) with an affinity constant of 7.34 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).

蛋白內(nèi)毒素:< 1.0 EU per μg of the protein as determined by the LAL method

預(yù)測(cè)N端:Gln 18

蛋白分子量:The recombinant human ACE2/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 961 amino acids and predicts a molecular mass of 110.3 kDa. As a result of glycosylation, the rhACE2/Fc monomer migrates as approximately 145-150 kDa band in SDS-PAGE under reducing conditions.

蛋白NP號(hào):NP_068576.1

蛋白氨基酸序列:Met1-Ser740

蛋白標(biāo)簽:C-human IgG1-Fc

歡迎去義翹神州網(wǎng)查看ACE2人重組蛋白，10108-H02H詳細(xì)信息。網(wǎng)址：https://cn.sinobiological.com/recombinant-proteins/human-ace2-10108-h02h

義翹神州研發(fā)團(tuán)隊(duì)擁有多年的重組蛋白研發(fā)經(jīng)驗(yàn)。多年來(lái)，我們的團(tuán)隊(duì)已開發(fā)出超過(guò)9300余種重組蛋白，涵蓋免疫檢查點(diǎn)、抗體藥物靶點(diǎn)、CAR-T細(xì)胞治療靶點(diǎn)、Fc受體、流感病毒蛋白以及細(xì)胞因子等熱門研究領(lǐng)域。義翹神州重組蛋白具有高純度、高生物活性和低內(nèi)毒素水平等特性，可為生物藥靶點(diǎn)發(fā)現(xiàn)、蛋白質(zhì)結(jié)構(gòu)和功能分析、細(xì)胞療法和重組酶酶學(xué)性質(zhì)等科學(xué)研究提供支持。

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